Prevalence of Multi Drug Resistant Pseudomonas Species Encoding SPM Metalo Beta Lactamase Gene Collected from Patients with Infected Wound in Khartoum State
Maha Assayed Abdullah Mohamed1 and Mohamed H. Arbab2*
1 University of Medical Sciences and Technology - Faculty of Medical Laboratory Science, Sudan.
2 Associate professor of medical Microbiology – Omdurman Ahlia University, Sudan.
*Corresponding Author: Mohamed H. Arbab, Associate professor of medical Microbiology – Omdurman Ahlia University, Sudan.
DOI: https://doi.org/10.58624/SVOAMB.2024.05.052
Received: August 17, 2024 Published: September 10, 2024
Abstract
Background: Selection of the most appropriate antibiotic is complicated by the ability of P. aeruginosa to develop resistance to multiple classes of antibacterial agents, even during the course of treating an infection. As in all genes encoding bacteria, metalloB-Lactamases-producing strains (generally multidrug resistant) cause infections that are difficult to treat and resulting in a high mortality and morbidity rates in the community, an increase in both to extend and diversity of metallo-β- lactamases in Pseudomonas aeruginosa severely limits treatment and delay the process of healing the wound of patients suffering from pseudomonal infection, extent the length of hospital stay and chronic care and overall cost of treating the infection, for this reason these study conducted to evaluate the presence of metallo B- lactamase gene encoding Pseudomonas species in Khartoum hospitals.
Aim: To detect Prevalence of Multi Drug Resistant Pseudomonas Species Encoding SPM Metalo Beta Lactamase Gene Collected from Patients with Infected Wound in Khartoum State.
Materials and method: The study was conducted in Khartoum state during the period from February to October 2018-09-22 the aim of the study was to evaluate the prevalence of SPM metalo B-lactamase gene produced by pseudomonades bacteria from infected wound, in Khartoum state. The study includes 380 wound swab collected from Khartoum hospitals. The study showed the prevalence rate of resistance against imipenem was 46 % and after carrying out the phenotypic experiments were identified as of MBL producer. Fourteen species were confirmed by Polymerase Chain Reaction (PCR) method showed positive for Gene SPM-1 among the positive (antibiotic resistant).
Result: A total 380 specimens250 males and 130 females with range from 20-75 years. (Figure-1), from which a total of 70 isolate were identified as Pseudomonas aeruginosa from different wound swab samples, collected from patient's attendant a different hospital in Khartoum, rate of resistance against Imipenem was 32(46 %), while the molecular detection of Metalo B –Lactamase encoding genes reveled 14(20%) Conclusion: This study concludes that Pseudomonas species that are considered as represent a serious therapeutic challenge for treatment of both community- acquired and nosocomial infections.
Keywords: Pseudomonas aeruginosa, metall-β-lactamase, antimicrobial resistance, wound infection, Sudan
Citation: Mohamed MAA, Arbab MH. Prevalence of Multi Drug Resistant Pseudomonas Species Encoding SPM Metalo Beta Lactamase Gene Collected from Patients with Infected Wound in Khartoum State. SVOA Microbiology 2024, 5:4, 143-147. doi:10.58624 SVOAMB.2024.05.052
