Examination of the prevalence of Escherichia coli containing the CTX-M-1 gene in urinary tract infection samples collected from Zanjan hospitals, Iran
Background and Aim: The increasing use of beta-lactam antimicrobials in the treatment of bacterial infections has increased resistance to them. One of the current problems in the treatment of nosocomial infections is the resistance of enzymes to broad-spectrum beta-lactamases (ESBL) among clinical isolates, especially Escherichia coli. Therefore, the aim of this study was to identify the molecular identification of CTX-M-1 genes in the E. coli strains isolated from urinary tract infections in Zanjan hospitals.
Materials and Methods: In this descriptive-analytical study of the study of 289 cases of urinary tract infection in Zanjan medical centers in 2019, 100 isolates of E. coli were identified by standard bacteriological methods. Antibiotic susceptibility of the isolates was determined by disk diffusion method and ESBL-producing isolates were identified by combined disk method. The bacterial DNA was then extracted and studied by PCR using specific gene primers.
Results: The most resistant to ampicillin (73%) and tetracycline (49%) were the most sensitive to amikacin (90%) and nitrofu-rantoin (89%), respectively. A total of 50 samples were identified as the final ESBL producer. 39 specimens of E. coli bacteria had the CTX-M-1 gene.
Conclusion: The genes studied in this study were all located on the chromosome of E. coli bacteria. Therefore, further inves-tigation of ESBL genes such as CTX-M-1 gene seems necessary to control this bacterium.
*Corresponding Author: Amir Pournajafi, Department of biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran, Email: amirpournajafi1990@gmail.com
Received: April 21, 2020 Published: April 27, 2020
Citation: Amir Pournajafi .et al. “Examination of the prevalence of Escherichia coli containing the CTX-M-1 gene in urinary tract infection samples collected from Zanjan hospitals, Iran”, SVOA Microbiology 1:3 (2020) 19-24.
Copyright: © 2020All rights reserved by Amir Pournajafi .et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
