Evaluation of New Glass Slide and Petri Dish Methods of Biofilm Testing in Uropathogenic Bacteria and Their Comparison with Test-Tube Method



Biswas M1, Bhattacharyya S2*, Banik A3 and Jilani G Md4

1 MSc. Biotechnology student, Swami Vivekananda Institute of Modern Science, Sonarpur, West Bengal.

2,3 Associate Professor, Microbiology, AIIH&PH Kolkata.

4 Professor, Department of Biotechnology, Swami Vivekananda Institute of Modern Science, Sonarpur, West Bengal.

*Corresponding Author: Bhattacharyya S, Associate Professor, Microbiology, AIIH&PH Kolkata.

DOI: https://doi.org/10.58624/SVOAMB.2023.04.029

Received: May 22, 2023     Published: June 26, 2023

 

Abstract

Biofilms are produced by many uropathogens and they render the drugs ineffective even if antibiotics are effective against the big in vivo. In this study we evaluated the biofilm forming potential of the uropathogenic bacteria by the test tube method and compared 2 new methods with it, which were the glass Petri dish method and glass slide method. We found that the glass petri dish method was as good as, if not better than Test tube method to detect biofilms in uropathogenic bacteria. The slide method was neither very sensitive specific. Also, biofilms in test tube method could be observed microscopically. So these can be explored further by researchers later on.

Keywords: Uropathogens, biofilm, slude, petri dish, test tube.

Citation: Biswas M, Bhattacharyya S, Banik, Jilani G Md. Evaluation of New Glass Slide and Petri Dish Methods of Biofilm Testing in Uropathogenic Bacteria and Their Comparison with Test-Tube Method. SVOA Microbiology 2023, 4:2, 35-41.