Biopigments Produced by Monascus purpureus Through Solid State Fermentation: HPTLC Finger Printing Analysis
Makhmur Ahmad1 and Bibhu Prasad Panda2*
1Department of Pharmaceutics, College of Dentistry and Pharmacy, Buraydah Private Colleges, Buraydah, Al-Qassim 31717, Kingdom of Saudi Arabia.
2Microbial and Pharmaceutical Biotechnology Laboratory, Center for Advanced Research in Pharmaceutical Sciences, School of Pharmaceutical Education and Research, Jamia Hamdard, New Delhi, India
*Corresponding Author: Dr. Bibhu Prasad Panda, (M.Pharm, Ph.D), Professor (Pharmaceutical Biotechnology), Microbial and Pharmaceutical Biotechnology Laboratory, Center for Advanced Research in Pharmaceutical Sciences, School of Pharmaceutical Education and Research, Jamia Hamdard, New Delhi, India.
DOI: https://doi.org/10.58624/SVOAMB.2023.04.024
Received: February 02, 2023 Published: February 20, 2023
Abstract
The current study aims to conduct HPTLC finger printing analysis of various extracts of Monascus purpureus biopigments (water extract, acetone extract, chloroform extract, acetonitrile extract, petroleum ether extract, methanol extract, ethanol extract, hexane extract). Natural and synthetic pigments both play an important roles as biocolourants in the food and pharmaceutical industries. The most recent HPTLC technique helps useful fingerprint analysis to determine the identity and composition of natural phytomolecules. Solid state fermentation was carried out with the help of a production medium contains long grain, unripe basmati rice. Water, acetone, chloroform, petroleum ether, acetonitrile, ethanol, methanol, and hexane were used to extract fermented rice (5g), which was then analysed at 485 nm. For scanning at different wavelengths of 370 nm (yellow pigment), 420 nm (red pigment), 470 nm (orange pigment), 500 nm, and 530 nm (red pigment), various mobile phases have been developed. When chromatographed with mobile phase 5 (ethyl acetate: isopropanol: water- 8.0:1.0:1.0 v/v), an acetone extract of Monascus biopigment showed good resolution.
Keywords: HPTLC, Monascus purpureus, Biopigments, Solid state fermentation, Finger printing analysis
Citation: Ahmad M, Panda BP. “Biopigments Produced by Monascus purpureus Through Solid State Fermentation: HPTLC Finger Printing Analysis”. SVOA Microbiology 2023, 4:1, 01-12.
